Circulation of human influenza viruses and emergence of Oseltamivir-resistant A(H1N1) viruses in Cameroon, Central Africa.
Identifieur interne : 000201 ( France/Analysis ); précédent : 000200; suivant : 000202Circulation of human influenza viruses and emergence of Oseltamivir-resistant A(H1N1) viruses in Cameroon, Central Africa.
Auteurs : Richard Njouom [Cameroun] ; Serge A Sadeuh Mba ; Dominique Noah Noah ; Victoria Gregory ; Patrick Collins ; Pierre Cappy ; Alan Hay [France] ; Dominique Rousset [Cameroun]Source :
- BMC Infectious Diseases [ 1471-2334 ] ; 2010.
Abstract
BACKGROUND: While influenza surveillance has increased in most developing countries in the last few years, little influenza surveillance has been carried out in sub-Saharan Africa and no information is available in Central Africa. The objective of this study was to assess the prevalence of influenza viruses circulating in Yaounde, Cameroon and determine their antigenic and genetic characteristics. METHODS: Throat and/or nasal swabs were collected from November 2007 to October 2008 from outpatients with influenza-like illness (ILI) in Yaounde, Cameroon and analyzed by two different techniques: a one-step real time reverse transcription-polymerase chain reaction (RT-PCR) and virus isolation in MDCK cells. Typing and subtyping of virus isolates was performed by hemagglutination inhibition (HI), and viruses were sent to the WHO Collaborating Centre in London, UK for further characterization and analyses of antiviral resistance by enzyme inhibition assay and nucleotide sequencing. RESULTS: A total of 238 patients with ILI were sampled. During this period 70 (29%) samples were positive for influenza by RT-PCR, of which only 26 (11%) were positive by virus isolation. By HI assay, 20 of the 26 isolates were influenza type A (10 H3N2 and 10 H1N1) and 6 were influenza type B (2 B/Victoria/2/87 lineage and 4 B/Yagamata/16/88 lineage). Seven (70%) of the H1N1 isolates were shown to be resistant to oseltamivir due to a H275Y mutation. CONCLUSIONS: This study confirmed the circulation of influenza A(H1N1), A(H3N2) and B viruses in the human population in Central Africa and describes the emergence of oseltamivir-resistant A(H1N1) viruses in Central Africa.
Url:
DOI: 10.1186/1471-2334-10-56
Affiliations:
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Hal:hal-00542166Le document en format XML
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Africa.</title><author><name sortKey="Njouom, Richard" sort="Njouom, Richard" uniqKey="Njouom R" first="Richard" last="Njouom">Richard Njouom</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-55917" status="VALID"> <orgName>Centre Pasteur du Cameroun</orgName> <desc> <address> <addrLine>B.P. 1274 Yaoundé</addrLine> <country key="CM"></country> </address> <ref type="url">http://www.pasteur-yaounde.org/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-301247" type="direct"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> 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1274 Yaoundé</addrLine> <country key="CM"></country> </address> <ref type="url">http://www.pasteur-yaounde.org/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-301247" type="direct"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Cameroun</country></affiliation></author></analytic><idno type="DOI">10.1186/1471-2334-10-56</idno><series><title level="j">BMC Infectious Diseases</title><idno type="ISSN">1471-2334</idno><imprint><date type="datePub">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> <p>BACKGROUND: While influenza surveillance has increased in most developing countries in the last few years, little influenza surveillance has been carried out in sub-Saharan Africa and no information is available in Central Africa. The objective of this study was to assess the prevalence of influenza viruses circulating in Yaounde, Cameroon and determine their antigenic and genetic characteristics. METHODS: Throat and/or nasal swabs were collected from November 2007 to October 2008 from outpatients with influenza-like illness (ILI) in Yaounde, Cameroon and analyzed by two different techniques: a one-step real time reverse transcription-polymerase chain reaction (RT-PCR) and virus isolation in MDCK cells. Typing and subtyping of virus isolates was performed by hemagglutination inhibition (HI), and viruses were sent to the WHO Collaborating Centre in London, UK for further characterization and analyses of antiviral resistance by enzyme inhibition assay and nucleotide sequencing. RESULTS: A total of 238 patients with ILI were sampled. During this period 70 (29%) samples were positive for influenza by RT-PCR, of which only 26 (11%) were positive by virus isolation. By HI assay, 20 of the 26 isolates were influenza type A (10 H3N2 and 10 H1N1) and 6 were influenza type B (2 B/Victoria/2/87 lineage and 4 B/Yagamata/16/88 lineage). Seven (70%) of the H1N1 isolates were shown to be resistant to oseltamivir due to a H275Y mutation. CONCLUSIONS: This study confirmed the circulation of influenza A(H1N1), A(H3N2) and B viruses in the human population in Central Africa and describes the emergence of oseltamivir-resistant A(H1N1) viruses in Central Africa.</p> </div></front></TEI><affiliations><list><country><li>Cameroun</li><li>France</li></country></list><tree><noCountry><name sortKey="Cappy, Pierre" sort="Cappy, Pierre" uniqKey="Cappy P" first="Pierre" last="Cappy">Pierre Cappy</name><name sortKey="Collins, Patrick" sort="Collins, Patrick" uniqKey="Collins P" first="Patrick" last="Collins">Patrick Collins</name><name sortKey="Gregory, Victoria" sort="Gregory, Victoria" uniqKey="Gregory V" first="Victoria" last="Gregory">Victoria Gregory</name><name sortKey="Mba, Serge A Sadeuh" sort="Mba, Serge A Sadeuh" uniqKey="Mba S" first="Serge A Sadeuh" last="Mba">Serge A Sadeuh Mba</name><name sortKey="Noah, Dominique Noah" sort="Noah, Dominique Noah" uniqKey="Noah D" first="Dominique Noah" last="Noah">Dominique Noah Noah</name></noCountry><country name="Cameroun"><noRegion><name sortKey="Njouom, Richard" sort="Njouom, Richard" uniqKey="Njouom R" first="Richard" last="Njouom">Richard Njouom</name></noRegion><name sortKey="Rousset, Dominique" sort="Rousset, Dominique" uniqKey="Rousset D" first="Dominique" last="Rousset">Dominique Rousset</name></country><country name="France"><noRegion><name sortKey="Hay, Alan" sort="Hay, Alan" uniqKey="Hay A" first="Alan" last="Hay">Alan Hay</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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